Which molecular diagnostic technique relies on fluorescently labeled probes that are cleaved by polymerase exonuclease activity during PCR to increase specificity?
Show answer and explanation
Correct answer: TaqMan qPCR assay
TaqMan qPCR uses a dual-labeled hydrolysis probe: during PCR, the Taq polymerase cleaves the probe if hybridized to target DNA, releasing fluorescence and providing highly specific quantitative detection.
Keep practicing
More Molecular diagnostics questions
- Which molecular diagnostic approach would best detect a small insertion or deletion (indel) mutation present at low allele frequency in a m…
- Which technique would you choose to detect a known chromosomal translocation in leukemia cells?
- What is a key advantage of isothermal amplification (e.g., LAMP or RPA) over conventional PCR in resource-limited settings?
- Which molecular diagnostic technique is most suitable for confirming presence of a viral RNA pathogen in a patient sample?
- In molecular diagnostics, what does high analytical sensitivity mean?
- Which molecular diagnostic method can provide information about both gene expression (mRNA levels) and possible gene mutations simultaneous…