Macrolides · Pharmacology

What is the primary mechanism of action of macrolide antibiotics?

  1. Inhibition of cell wall synthesis by binding to PBPs
  2. Binding to the 30S ribosomal subunit to cause mRNA misreading
  3. Binding to the 50S ribosomal subunit to inhibit protein synthesis
  4. Inhibition of DNA gyrase to prevent DNA replication
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Correct answer: Binding to the 50S ribosomal subunit to inhibit protein synthesis

Macrolides inhibit bacterial protein synthesis by reversibly binding to the 23S rRNA of the 50S ribosomal subunit. This action blocks the translocation step of elongation, preventing the movement of the peptidyl-tRNA from the A site to the P site.

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