Fluoroquinolones · Pharmacology

Which of the following describes the primary mechanism of action of fluoroquinolones in Gram-negative bacteria?

  1. Inhibition of RNA polymerase
  2. Inhibition of DNA gyrase (Topoisomerase II)
  3. Binding to the 50S ribosomal subunit
  4. Disruption of the peptidoglycan cross-linking
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Correct answer: Inhibition of DNA gyrase (Topoisomerase II)

In Gram-negative bacteria, the primary target of fluoroquinolones is DNA gyrase. By inhibiting this enzyme, the drugs prevent the relaxation of positively supercoiled DNA that is required for normal transcription and replication.

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