Which of these enzymes is 'thermostable' and used specifically in some one-step RT-PCR kits to allow higher RT temperatures?
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Correct answer: Tth DNA Polymerase
Tth DNA polymerase is a unique enzyme that possesses both reverse transcriptase and DNA polymerase activities in the presence of manganese. Its thermostability allows reverse transcription to occur at high temperatures, which helps overcome complex RNA secondary structures.
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More RT-PCR questions
- What is the primary function of the 'Reverse Transcriptase' enzyme in an RT-PCR reaction?
- In the context of RT-PCR, what is the specific purpose of using 'Oligo(dT)' primers during the reverse transcription step?
- Which of the following describes 'One-step' RT-PCR?
- What is the clinical significance of a 'One-step' RT-PCR assay in the detection of RNA viruses like SARS-CoV-2?
- When performing RT-PCR, why is it often necessary to treat the RNA sample with DNase I prior to reverse transcription?
- Which type of primer is most appropriate for reverse transcribing prokaryotic (bacterial) RNA or highly degraded RNA samples?