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RT-PCR · Pathology

Which of the following describes 'One-step' RT-PCR?

  1. Reverse transcription and PCR occur in a single reaction tube
  2. The RNA is extracted and amplified without the need for cDNA synthesis
  3. Only one primer is used for both transcription and amplification
  4. The reaction is completed in exactly one thermal cycle
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Correct answer: Reverse transcription and PCR occur in a single reaction tube

One-step RT-PCR combines reverse transcription and PCR in a single reaction vessel using a specialized buffer and enzyme mix. This reduces handling time and the risk of contamination compared to two-step protocols.

Difficulty: Medium Question 3 of 20

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