In the context of PCR contamination, what is the role of Uracil-N-Glycosylase (UNG)?
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Correct answer: To degrade prior PCR products containing dUTP
UNG is used in a pre-PCR incubation step to eliminate 'carry-over' contamination. It cleaves uracil from DNA strands produced in previous runs (where dUTP was substituted for dTTP), preventing them from acting as templates.
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