Which of the following modifications is used in Allele-Specific PCR to detect a Single Nucleotide Polymorphism (SNP)?
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Correct answer: Designing primers where the 3' terminal base matches the specific SNP
DNA polymerase requires a perfectly matched 3' end to initiate extension. By placing the SNP site at the 3' end of the primer, amplification will only occur if the specific allele is present in the sample.
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