In the clinical laboratory, 'Clean Room' protocols for PCR involve which of the following?
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Correct answer: Using a unidirectional workflow from Pre-PCR to Post-PCR areas
To prevent contamination of patient samples with amplified DNA (amplicons), Pre-PCR tasks (mix prep, DNA extraction) must be physically separated from Post-PCR tasks. Personnel and equipment should not move from Post-PCR back to Pre-PCR.
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