Which component of a TaqMan probe is responsible for suppressing fluorescence when the probe is intact?
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Correct answer: Non-fluorescent quencher
In a TaqMan probe, a reporter dye at the 5' end and a quencher at the 3' end are in close proximity. The quencher absorbs the reporter's signal through FRET until the probe is cleaved by the polymerase's 5' exonuclease activity.
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