Why is bisulfite sequencing specifically utilized in epigenetic studies?
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Correct answer: It selectively converts unmethylated cytosines to uracil
Treatment of DNA with sodium bisulfite deaminates unmethylated cytosine residues to uracil (read as thymine after PCR), while 5-methylcytosine is resistant to this conversion. Comparing bisulfite-treated sequences to reference sequences reveals the methylation status at single-base resolution.
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