A researcher is studying a pathogen with a high AT-rich genome. Which sequencing technology is generally considered most challenging for this type of genome?

Correct answer: Illumina sequencing-by-synthesis

Illumina sequencing often struggles with AT-rich genomes due to lower complexity and amplification bias during bridge PCR. This can lead to uneven coverage and drop-out in these regions, whereas long-read technologies like PacBio and Nanopore are less susceptible to this specific amplification bias.