Which quality control metric is most indicative of successful library preparation for NGS?
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Correct answer: The size distribution of the fragmented DNA library
After fragmentation and adapter ligation, the library should have a specific size distribution (e.g., 300-500 bp for Illumina). Checking the fragment size using a Bioanalyzer or TapeStation ensures that the library is properly constructed and free of excessive adapter dimers or large concatemers.
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