In next-generation sequencing (NGS), what is the primary function of bridge amplification?
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Correct answer: To create clonal clusters of DNA fragments on a flow cell
Bridge amplification is a process used in Illumina sequencing platforms to generate clonal clusters. Adapter-ligated DNA fragments hybridize to oligonucleotides on the flow cell and are amplified into distinct, spatially separated clusters, which enhances the signal-to-noise ratio during the sequencing-by-synthesis step.
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