Which of the following fixatives is best suited for preserving lipids?
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Correct answer: Osmium tetroxide
Routine formalin processing dissolves lipids during the dehydration and clearing stages. Osmium tetroxide chemically reacts with and fixes lipids, making it essential for electron microscopy and myelin studies.
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More Tissue Processing questions
- What is the primary hazard associated with the use of Xylene in the lab?
- What is the recommended ratio of fixative volume to tissue volume for effective fixation?
- Which processing step is specifically required for specimens like bone or teeth before routine processing?
- What is the effect of 'over-fixation' in formalin?
- Which reagent is used as a 'secondary fixative' for electron microscopy to provide high contrast?
- What is the primary advantage of using a vacuum during tissue processing?