What is the standard thickness for routine Hematoxylin and Eosin (H&E) stained paraffin sections?
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Correct answer: 3–5 micrometers
Routine paraffin sections are usually cut at 3–5 micrometers to ensure a single layer of cells for clear microscopic visualization. Thicker sections result in overlapping nuclei, while thinner sections may lack sufficient staining intensity.
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