Microtomy · Pathology

What is the primary cause of 'chatter' or fine parallel lines appearing on a tissue section?

  1. Paraffin wax that is too soft
  2. Dull knife or loose knife/block clamping
  3. Excessive clearance angle
  4. Water bath temperature being too high
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Correct answer: Dull knife or loose knife/block clamping

Chatter is caused by vibration during the cutting stroke, often due to the knife or the specimen block not being clamped securely. It can also occur if the tissue is extremely hard or if the clearance angle is excessive.

Difficulty: Medium Question 4 of 20

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