Histopathology

Microtomy Practice Questions

20 free Microtomy practice questions for the Pathology, each with the correct answer and a detailed explanation. Open any question below, or take the full set as an interactive quiz.

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Questions

All Microtomy questions

20 questions
  1. Q1. Which type of microtome is most commonly used in routine histopathology laboratories for sectioning paraffin-embedded blocks?
  2. Q2. What is the standard thickness for routine Hematoxylin and Eosin (H&E) stained paraffin sections?
  3. Q3. Which of the following describes the 'clearance angle' in microtomy?
  4. Q4. What is the primary cause of 'chatter' or fine parallel lines appearing on a tissue section?
  5. Q5. In a cryostat, which of the following is used to rapidly freeze tissue specimens for intraoperative consultation?
  6. Q6. What is the ideal temperature for a flotation water bath used to expand paraffin sections?
  7. Q7. Which defect is characterized by sections varying in thickness (thick and thin sections) or skipping sections entirely?
  8. Q8. When sectioning extremely hard tissues like decalcified bone or uterus, which type of knife edge is most appropriate?
  9. Q9. Which of the following will result in 'scored' or 'split' sections where a line runs through the entire ribbon?
  10. Q10. What is the purpose of 'trimming' or 'facing' the block before taking actual sections?
  11. Q11. Which additive is commonly used in a water bath to help sections adhere better to the glass slide?
  12. Q12. What is the typical thickness required for 'ultra-thin' sections in electron microscopy?
  13. Q13. A technician notices that the paraffin ribbon is curving to one side. What is the most likely cause?
  14. Q14. Which of the following is a disadvantage of using frozen sections (cryostat) compared to paraffin sections?
  15. Q15. What is 'compression' in microtomy, and how is it usually corrected?
  16. Q16. Which of the following is used to maintain a constant low temperature for sectioning fresh tissue in a cryostat?
  17. Q17. What is the effect of 'parched' or 'explosive' sections on a water bath?
  18. Q18. Which instrument is used to produce extremely thin sections (0.5 to 1 micrometer) often referred to as 'semi-thin' or 'thick' sections for light microscopy of…
  19. Q19. What is 'static electricity' in microtomy, and how does it affect the process?
  20. Q20. Which of the following describes the 'bevel angle' of a microtome knife?