Artifacts in Histology · Pathology

Tissue shrinkage during processing is most often due to:

  1. Inadequate tissue dehydration
  2. Prolonged fixation in formalin
  3. Excessive dehydration with alcohol
  4. Improper staining technique
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Correct answer: Excessive dehydration with alcohol

Excessive dehydration during processing causes tissue shrinkage and separation artifacts. Alcohol removes water aggressively, leading to distortion of tissue architecture.

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