Artifacts in Histology · Pathology

Formalin pigment (acid hematin) is most likely to appear in tissues fixed with:

  1. Neutral buffered formalin
  2. Unbuffered acidic formalin
  3. Alcohol-based fixatives
  4. Glutaraldehyde
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Correct answer: Unbuffered acidic formalin

Formalin pigment forms in acidic formalin solutions. It appears as dark brown deposits and can be mistaken for pathological pigment.

Difficulty: Medium Question 4 of 20

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