Artifacts in Histology · Pathology

Knife lines seen across a histologic section are typically caused by:

  1. Improper fixation
  2. Blunt or damaged microtome blade
  3. Overstaining with hematoxylin
  4. Improper coverslipping
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Correct answer: Blunt or damaged microtome blade

Knife lines result from a dull or nicked microtome blade. These lines appear as parallel streaks across the tissue section.

Difficulty: Medium Question 3 of 20

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