Interstitial Growth · Histology

Which histological stain is most effective for visualizing the intense basophilia around an isogenous group during active interstitial growth?

  1. Toluidine Blue or Alcian Blue
  2. Verhoeff's Elastic Tissue stain
  3. Sudan Black B or Oil Red O
  4. Periodic Acid-Schiff stain
Show answer and explanation

Correct answer: Toluidine Blue or Alcian Blue

Active chondrocytes secrete territorial matrix rich in glycosaminoglycans (GAGs). Stains like Toluidine Blue exhibit metachromasia, staining these areas intensely due to the high density of sulfate groups in the new matrix.

Difficulty: Medium Question 10 of 20

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