Microscopy Resolution · Histology

Empty magnification occurs when:

  1. The image is enlarged beyond the resolving power of the objective lens
  2. The resolution of the image is improved without increasing magnification
  3. The numerical aperture is too high for the light source
  4. The specimen is not stained properly
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Correct answer: The image is enlarged beyond the resolving power of the objective lens

Empty magnification refers to increasing the size of an image (e.g., using a 20x ocular) without revealing any new detail. This happens when the magnifying power exceeds the limit set by the objective's resolution.

Difficulty: Medium Question 8 of 20

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