Transcription and Translation · USMLE Step 1

Which RNA polymerase transcribes tRNA and 5S rRNA in eukaryotic cells?

  1. Eukaryotic RNA polymerase I
  2. Eukaryotic RNA polymerase II
  3. Eukaryotic RNA polymerase III
  4. Eukaryotic RNA polymerase IV
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Correct answer: Eukaryotic RNA polymerase III

In eukaryotes, RNA polymerase III transcribes tRNAs, 5S rRNA, and some small nuclear RNAs important for splicing, while RNA polymerase II transcribes pre-mRNAs.

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