Transcription and Translation · USMLE Step 1

Which of these processes occur before export of a newly transcribed mRNA from the nucleus in eukaryotes?

  1. Cytoplasmic translation initiation on the mRNA
  2. 5' capping, intron splicing, and polyadenylation
  3. tRNA charging by aminoacyl synthetases
  4. Assembly of the 80S ribosomal subunits
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Correct answer: 5' capping, intron splicing, and polyadenylation

Before export to the cytoplasm, eukaryotic pre-mRNA undergoes 5′ capping, intron splicing, and 3′ poly-A tail addition to become mature mRNA. Only mature mRNA can be exported and translated.

Difficulty: Medium Question 10 of 22

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