Transcription and Translation · USMLE Step 1

What is the role of aminoacyl-tRNA synthetase in translation?

  1. It catalyzes peptide-bond formation between amino acids on the ribosome
  2. It attaches the correct amino acid to its cognate tRNA, consuming ATP
  3. It unwinds mRNA secondary structure prior to small-subunit ribosome binding
  4. It excises non-coding introns from eukaryotic pre-mRNA before export
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Correct answer: It attaches the correct amino acid to its cognate tRNA, consuming ATP

Aminoacyl-tRNA synthetases catalyze the esterification of a specific amino acid to its corresponding tRNA using ATP, producing charged tRNA for translation.

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