What distinguishes third-generation sequencing (e.g., Oxford Nanopore) from second-generation sequencing (e.g., Illumina)?
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Correct answer: It sequences single molecules in real time without amplification
Third-generation sequencing technologies sequence single DNA molecules in real time without the need for PCR amplification. This enables ultra-long reads and direct detection of base modifications, albeit currently with higher raw error rates than second-generation sequencing.
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