Microtomy · Pathology

Which of the following is used to maintain a constant low temperature for sectioning fresh tissue in a cryostat?

  1. A liquid nitrogen immersion bath
  2. A refrigerated cabinet enclosing a built-in microtome
  3. Dry ice packed tightly around an enclosed sliding microtome
  4. An electric vacuum pressure pump
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Correct answer: A refrigerated cabinet enclosing a built-in microtome

A cryostat is essentially a rotary microtome housed within a refrigerated chamber, usually maintained between -15°C and -30°C. This allows for the sectioning of tissue while it remains in a frozen state.

Difficulty: Medium Question 16 of 20

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