Microtomy · Pathology

Which of the following is a disadvantage of using frozen sections (cryostat) compared to paraffin sections?

  1. Much slower turnaround time for intraoperative diagnoses
  2. Poorer morphological detail and freeze artifact
  3. Inability to reliably perform immunohistochemistry
  4. Requirement for toxic and hazardous clearing agents
Show answer and explanation

Correct answer: Poorer morphological detail and freeze artifact

Frozen sections prioritize speed over quality; ice crystal formation can distort cellular architecture (freeze artifact). Paraffin sections provide superior preservation of detail but take much longer to process.

Difficulty: Medium Question 14 of 20

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