Which stain is used in the Papanicolaou technique to provide the characteristic green/blue color to the cytoplasm of intermediate cells?
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Correct answer: Light Green (EA-50 formula)
The Papanicolaou stain uses Hematoxylin for the nucleus and two counterstains for the cytoplasm: Orange G and Eosin Azure (EA). Light Green, a component of EA, stains the cytoplasm of metabolically active cells, such as intermediate and parabasal cells, green or blue.
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