During the dehydration step of glycolysis, 2-phosphoglycerate is converted to phosphoenolpyruvate (PEP) by which enzyme?
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Correct answer: Enolase
Enolase catalyzes the removal of a water molecule from 2-phosphoglycerate to form the high-energy compound phosphoenolpyruvate (PEP). This step prepares the molecule for the final ATP-generating reaction.
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