Which step in glycolysis involves the use of inorganic phosphate (Pi) rather than ATP?
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Correct answer: PGAL → 1,3-BPGA (using Pi)
The conversion of glyceraldehyde-3-phosphate (PGAL) to 1,3-bisphosphoglycerate (BPGA) uses inorganic phosphate from the cytosol. This is an oxidation-coupled phosphorylation step.
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