Tissue Preparation · Histology

What occurs during the 'infiltration' step of tissue processing?

  1. The tissue is saturated with molten paraffin wax
  2. The tissue is washed thoroughly in running tap water
  3. The tissue is exposed to hematoxylin dye
  4. The tissue is frozen in liquid nitrogen
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Correct answer: The tissue is saturated with molten paraffin wax

Infiltration involves placing the cleared tissue into warm, liquid paraffin. The wax fills the spaces previously occupied by the clearing agent, providing internal support for the tissue during sectioning.

Difficulty: Medium Question 7 of 20

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