Microscopy Resolution · Histology

Which of the following would DECREASE the resolution of a histological image?

  1. Moving from a 40x to a 100x (oil) objective
  2. Closing the condenser diaphragm excessively
  3. Using a shorter wavelength filter
  4. Applying immersion oil to an oil-immersion lens
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Correct answer: Closing the condenser diaphragm excessively

Closing the condenser diaphragm reduces the cone of light entering the objective, which effectively lowers the Numerical Aperture of the system. While it increases contrast, it degrades the resolution.

Difficulty: Medium Question 12 of 20

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