Immunohistochemistry · Histology

Which of the following best describes a positive immunohistochemical staining result?

  1. The tissue shows uniform blue staining without variation
  2. No visible color change is observed within the tissue
  3. A visible colored deposit appears where antigen is present
  4. The entire tissue section dissolves completely during processing
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Correct answer: A visible colored deposit appears where antigen is present

Positive staining occurs when the enzyme–chromogen reaction produces a colored precipitate at sites where the antigen is present. This indicates successful antibody binding.

Difficulty: Medium Question 18 of 20

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