Immunohistochemistry · Histology

What is the role of a secondary antibody in many immunohistochemistry techniques?

  1. To enzymatically digest tissue proteins before fixation
  2. To bind directly to DNA within the tissue section
  3. To bind to the primary antibody and amplify the signal
  4. To dissolve tissue sections during the staining process
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Correct answer: To bind to the primary antibody and amplify the signal

Secondary antibodies bind to primary antibodies and are usually conjugated with enzymes or fluorophores. This amplifies the signal and allows visualization of the antigen–antibody complex.

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