Which chromogen is commonly used with horseradish peroxidase in immunohistochemistry?
Show answer and explanation
Correct answer: Diaminobenzidine
Diaminobenzidine (DAB) reacts with horseradish peroxidase to produce a brown precipitate at the site of antigen–antibody binding. This color allows pathologists to identify positive staining areas in tissue sections.
Keep practicing
More Immunohistochemistry questions
- What is antigen retrieval in immunohistochemistry?
- Which fixative is most commonly used before immunohistochemistry processing?
- Which microscopic technique is typically used to observe immunohistochemical staining with enzyme-based chromogens?
- What is the purpose of blocking steps in immunohistochemistry?
- Which type of antibody is applied first to bind directly to the target antigen in immunohistochemistry?
- Which of the following best describes a monoclonal antibody?