Plant Tissue Culture · Botany

Which of the following describes the 'Vitrification' (or Hyperhydricity) physiological disorder in culture?

  1. Excessive woodiness of the stem tissue
  2. Glassy, water-soaked appearance of tissues
  3. Premature flowering of the plantlet
  4. Total loss of chlorophyll pigmentation in leaves
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Correct answer: Glassy, water-soaked appearance of tissues

Vitrification occurs due to high humidity and low light in the culture vessel, leading to abnormal stomata and poor cuticle development. This results in brittle, translucent shoots that often fail to survive acclimatization.

Difficulty: Medium Question 11 of 20

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