Plant Tissue Culture · Botany

What is 'Hardening' in the context of plant tissue culture?

  1. The process of making the semi-solid agar culture medium firmer and more stable
  2. The gradual accumulation of lignin in the callus tissue over time
  3. The sterilization of glass culture vessels using prolonged dry heat
  4. The gradual acclimatization of in vitro plants to the natural environment
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Correct answer: The gradual acclimatization of in vitro plants to the natural environment

Plants grown in vitro have thin cuticles and non-functional stomata. Hardening involves gradually exposing them to lower humidity and higher light intensity to prepare them for soil-based survival.

Difficulty: Medium Question 16 of 20

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