The 'isoelectric point' (pI) of an amino acid is the pH at which:
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Correct answer: The amino acid carries no net electrical charge
The isoelectric point (pI) is the pH at which an amino acid exists as a zwitterion — the positive charge on the protonated amino group exactly balances the negative charge on the deprotonated carboxyl group, yielding zero net charge. At this pH the molecule will not migrate in an electric field.
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