Infection control principles · USMLE Step 1

Which method is most effective at inactivating bacterial spores on surgical instruments?

  1. Alcohol-based surface disinfectant
  2. Prolonged boiling in water
  3. Autoclaving under pressure
  4. Ultraviolet light exposure
Show answer and explanation

Correct answer: Autoclaving under pressure

Autoclaving uses high pressure and temperature to reliably destroy all microorganisms, including resistant spores. Other methods are insufficient for sterilization.

Difficulty: Medium Question 2 of 20

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