Immunohistochemistry · Pathology

Which microscopic technique is typically used to observe immunohistochemical staining with enzyme-based chromogens?

  1. Transmission electron microscopy
  2. Bright-field light microscopy
  3. Phase contrast microscopy
  4. Confocal fluorescence microscopy
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Correct answer: Bright-field light microscopy

Chromogenic reactions such as those producing brown DAB precipitate are visible under standard bright-field light microscopy. This allows pathologists to easily examine stained tissues.

Difficulty: Medium Question 9 of 20

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