Immunohistochemistry · Pathology

Which fixative is most commonly used before immunohistochemistry processing?

  1. Acetone
  2. Formalin
  3. Methanol
  4. Ethanol
Show answer and explanation

Correct answer: Formalin

Formalin fixation preserves tissue architecture and proteins by forming cross-links. However, these cross-links can mask antigen sites, which is why antigen retrieval steps are often required.

Difficulty: Medium Question 8 of 20

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