A pathology resident observes a brown, granular pigment in a blood-rich tissue section fixed in unbuffered formalin. This pigment can be removed by treating the slide with:
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Correct answer: Picric acid-alcohol solution
Acid formaline pigment (hemain) forms when formalin pH drops below 6.0 and reacts with hemoglobin. It can be removed using saturated alcoholic picric acid or Kardasewitsch's method.
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