Stool Examination · Pathology

What is the primary purpose of adding 'Lugol's Iodine' to a wet mount of stool during microscopic examination?

  1. To immobilize motile trophozoites for cell counting
  2. To highlight internal structures of protozoan cysts
  3. To detect and confirm the presence of occult blood
  4. To dissolve and clear vegetable fibers in stool
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Correct answer: To highlight internal structures of protozoan cysts

Iodine is a vital stain for protozoan cysts, highlighting the number and arrangement of nuclei and the presence of glycogen vacuoles. While it stains cysts clearly, it unfortunately kills motile trophozoites, necessitating a saline mount for motility assessment.

Difficulty: Medium Question 7 of 20

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