Stool Examination · Pathology

The 'Apt Test' (fecal hemoglobin alkali denaturation test) is used in neonates to distinguish between:

  1. Bacterial and viral diarrhea causes
  2. Fetal blood and swallowed maternal blood
  3. Conjugated and unconjugated bilirubin forms
  4. Fat content and mucus in the stool
Show answer and explanation

Correct answer: Fetal blood and swallowed maternal blood

The Apt test utilizes the fact that fetal hemoglobin (HbF) is resistant to alkali (NaOH) denaturation, while adult hemoglobin (HbA) is not. If the stool/vomit remains pink after adding alkali, it indicates the presence of HbF, implying the blood belongs to the neonate.

Difficulty: Medium Question 10 of 20

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