Adipocytes · Histology

Why do adipocytes appear as empty, clear spaces in routine histological preparations?

  1. They contain no organelles or cytoplasm.
  2. The cells are naturally transparent to light.
  3. The stored lipid is dissolved by organic solvents used in tissue processing.
  4. They contain a water-soluble carbohydrate that is washed away during fixation.
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Correct answer: The stored lipid is dissolved by organic solvents used in tissue processing.

Standard histological processing involves dehydration with alcohols and clearing with solvents like xylene or histoclear. These chemicals dissolve the triglycerides, leaving behind only the thin cellular borders.

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