Histological Staining Techniques · Histology

Which property of dyes determines whether they stain acidic or basic tissue components?

  1. Charge of the dye molecule
  2. Visible color of the dye
  3. Temperature of the staining solution
  4. Size of the dye molecule
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Correct answer: Charge of the dye molecule

The electrical charge of the dye determines its affinity for tissue components. Basic dyes bind acidic structures like nucleic acids, while acidic dyes bind basic proteins.

Difficulty: Medium Question 15 of 20

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