Histological Staining Techniques · Histology

Why is counterstaining often used in histological staining procedures?

  1. To remove excess primary dye from the section
  2. To provide contrast to the primary stain
  3. To dissolve lipids
  4. To increase tissue thickness
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Correct answer: To provide contrast to the primary stain

Counterstaining provides contrast to highlight structures not stained by the primary dye. For example, eosin serves as a counterstain to hematoxylin in H&E staining.

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