Histological Staining Techniques · Histology

What is metachromasia in histological staining?

  1. Staining limited exclusively to nuclear regions
  2. Total failure of dye to bind any tissue
  3. A color shift in the dye upon tissue binding
  4. Uniform dye uptake across every cell type
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Correct answer: A color shift in the dye upon tissue binding

Metachromasia refers to a phenomenon where a dye changes color when it binds to certain substances, such as cartilage matrix or mast cell granules. This occurs due to chemical interactions between the dye and tissue components.

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