Electron Microscopy · Histology

Which artifact is caused by the high-energy electron beam damaging or 'burning' a thin section during viewing?

  1. Beam damage
  2. Chatter
  3. Knife lines
  4. Formalin pigment
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Correct answer: Beam damage

If the electron beam is too intense or focused on one spot for too long, it can vaporize the resin or specimen. This results in holes or distorted areas in the image known as beam damage.

Difficulty: Medium Question 14 of 20

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